Modern DNA techniques applied to nineteenth-century potatoes

ScienceDaily (Sep. 19, 2012) ? Researchers led by Professor Bruce Fitt, now at the University of Hertfordshire, have used modern DNA techniques on late nineteenth-century potatoes to show how the potato blight may have survived between cropping seasons after the Irish potato famine of the 1840s.

Late blight of potato is caused by the microorganism, Phytophthora infestans, which rapidly destroys the leaves of potato crops and was responsible for the infamous Irish potato famine of the 1840s that left over one million people dead and another one million Irish emigrating. With growing concerns over food shortages and climate change, late blight remains a serious disease problem in current potato production and has also emerged as a significant disease threat to the organic tomato industry.

In the research paper published in Plant Pathology, DNA was extracted from the Rothamsted potato samples that had been dried, ground and stored in glass bottles in the nineteenth century. The DNA was then analysed for the presence of the potato blight pathogen.

Bruce Fitt, Professor of Plant Pathology at the University of Hertfordshire and formerly at Rothamsted Research, said: "It was the foresight of two nineteenth-century plant scientists to archive potato samples from their experiment that has enabled us to apply modern DNA techniques to better understand late potato blight and the implications for today's food security. The analysis of these late nineteenth-century potato samples is the earliest proof of how this disease survived between seasons in England."

The findings of this research has proved that the DNA technique applied to the potato samples is a very useful tool in plant disease diagnosis to test seed potatoes or tomato transplants for the presence of the late blight pathogen. This technique can be further developed for testing for other diseases found in different plants which affect food production.

Bruce continued: "Using modern DNA techniques to detect and quantify the pathogen in potatoes enables us to better understand the spread of potato late blight. This disease is still a serious threat to worldwide potato production."

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Story Source:

The above story is reprinted from materials provided by University of Hertfordshire, via AlphaGalileo.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.

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Journal Reference:

1. J. B. Ristaino, C. H. Hu, B. D. L. Fitt. Evidence for presence of the founder Ia mtDNA haplotype of Phytophthora infestans in 19th century potato tubers from the Rothamsted archives. Plant Pathology, 2012; DOI: 10.1111/j.1365-3059.2012.02680.x

Note: If no author is given, the source is cited instead.

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Source: http://feeds.sciencedaily.com/~r/sciencedaily/~3/voQn23516Vs/120919083403.htm

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